2018-02-16 12:11:042025-05-28 02:06:29
description
class B penicillin-binding protein PBP 2A, required for cell wall synthesis during cell elongation
class B penicillin-binding protein PBP 2A, required for [SW|cell wall synthesis] during cell elongation, part of the [[protein|Rod complex]] for lateral [SW|cell wall synthesis] and control of cell diameter
locus
BSU25000
BSU_25000
geneLength
2148
2151
outlinks
bsu
BSU25000
BSU_25000
Gene
Coordinates
2,581,771 → 2,583,921
2,581,771 2,583,921
The protein
[SW|Localization]
extracellular (signal peptide) [Pubmed|18957862]
localization depends on the availability of peptidoglyan precursors (lipid II) [Pubmed|23895585]
extracellular (signal peptide) [Pubmed|18957862]
during vegetative growth: evenly distributed along the membrane [pubmed|14731276]
localization depends on the availability of peptidoglyan precursors (lipid II) [Pubmed|23895585]
Biological materials
Mutant
MGNA-C461 (yqgF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2459 NBRP B. subtilis, Japan]
BKE25000 (Δ[[gene|pbpA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACATCACCTTTTCTAT, downstream forward: _UP4_TAAAAAAAAACGCTCACATG
BKK25000 (Δ[[gene|pbpA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACATCACCTTTTCTAT, downstream forward: _UP4_TAAAAAAAAACGCTCACATG
MGNA-C461 (yqgF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2459 NBRP B. subtilis, Japan]
BKE25000 ([[gene|pbpA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACATCACCTTTTCTAT, downstream forward: _UP4_TAAAAAAAAACGCTCACATG
BKK25000 ([[gene|pbpA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACATCACCTTTTCTAT, downstream forward: _UP4_TAAAAAAAAACGCTCACATG
Labs working on this gene/protein
[SW|Jeff Errington], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/jeff.errington homepage]
References
The protein
Protein family
[SW|transpeptidase family] (according to UniProt)
The protein
Paralogous protein(s)
[[protein|PbpH]]
Biological materials
GFP fusion
3103 [[gene|trpC]]2 [[gene|pbpA]]::pSG5043 (cat Pxyl-gfpa-[[gene|pbpA]]1-804) [pubmed|14731276], available in [SW|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]
labs
[SW|Jeff Errington], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/jeff.errington homepage]